Distinctive Features of Surface-Anchored Proteins of Streptococcus agalactiae Strains from Zimbabwe Revealed by PCR and Dot Blotting

Distinctive features of surface-anchored proteins of Streptococcus agalactiae strains from Zimbabwe revealed by PCR and dot blotting.

The distribution of capsular polysaccharide (CPS) types and subtypes (serovariants) among 121 group B streptococcus (GBS) strains from Zimbabwe was examined. PCR was used for the detection of both CPS types and the surface-anchored and strain-variable proteins Calpha, Cbeta, Alp1, Alp2, Alp3, R4/Rib, and Alp4. The R3 protein was detected by an antibody-based method using monoclonal anti-R3 anti...

Type determination by PCR and Dot Blotting of Streptococcus agalactiae 1 Strains from Zimbabwe Revealed Distinctive Features of Surface - Anchored 2 Proteins 3 4

4 Rooyen T. Mavenyengwa 1 , Johan A. Maeland 2* , Sylvester R. Moyo 3 5 6 7 Department of Medical Microbiology, College of Health Sciences, University of Zimbabwe 1 , 8 Department of Laboratory Medicine, Children’s and Women’s Health, St Olav Hospital, 9 Norwegian University of Science and Technology (NTNU) 2 , Department of Life and Consumer 10 Sciences, School of Agriculture and Life Sciences...

The Comparison of Proteins Elaborated by Streptococcus mutans Strains Isolated from Caries Free and Susceptible Subjects

Identification of major outer surface proteins of Streptococcus agalactiae.

To identify the major outer surface proteins of Streptococcus agalactiae (group B streptococcus), a proteomic analysis was undertaken. An extract of the outer surface proteins was separated by two-dimensional electrophoresis. The visualized spots were identified through a combination of peptide sequencing and reverse genetic methodologies. Of the 30 major spots identified as S. agalactiae speci...

Evaluation of Echinococcus granulosus coproantigens by Dot-blotting in dogs

Definitive hosts of the Echinococcus granulosus (E. granulosus ) parasite are carnivores such as dogs,wolves and foxes. Detection of this parasite through faecal examination is not possible. In this study, dotblotting test for E. granulosus -specific coproantigens has been evaluated in dog. Three 2–3-month-old puppies were treated with piperazine and then faecal samples were collected as pre-in...